Library-free methylation sequencing with bisulfite padlock probes.

Diep D, Plongthongkum N, Gore A, Fung HL, Shoemaker R, Zhang K.

Nat Methods. 2012 Feb 5;9(3):270-2.

Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
<<<<<<< HEAD ======= >>>>>>> Fix publications statics loading