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Roadmap Epigenome Project Protocols Database
Methylome Analysis
DNaseI
ChIP-Seq
RNA Analysis
General Protocols
Data
Standards
REMC Standards and Guidelines for ChIP-seq
v1.0
REMC Standards and Guidelines for RNA-sequencing
DRAFT v3.0
Standards and Guidelines for Whole Genome Shotgun Bisulfite Sequencing
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Experimental
Protocols
5xMRE-seq library construction protocol v5
November 2010 -- Costello Lab
ChIP Library Preparation Protocol
Ren Lab
ChIP Protocol for Full REMC (6 marks)
Broad
ChIP-Seq Protocol: Cells
UW Stam Lab
ChIP-Seq Protocol: Tissue samples
UW Stam Lab
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General Protocols protocols
Crosslinking Adherent Cell Lines
BC
Download this protocol here
Crosslinking of Frozen Cell Pellet
BC
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Cryopreservation of human hematopoietic cells
UW Stam Lab
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DNA Sonication using Sonic Dismembrator 550
BC
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Lymphoid cells from apheresis, Freezing
UW Stam Lab
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Lymphoid cells from apheresis, Slow Thawing
UW Stam Lab
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Operation of the Covaris E-series
BC
Download this protocol here
Slow thawing of cryopreserved hematopoietic cells from human leukapheresis product
UW Stam Lab
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SLX-PET Protocol for Illumina Sample Prep
BC
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Small Amount DNA Solexa Library Construction – Aug 2009
Broad
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Solexa Indexed Paired-End Library Construction
UW Stam Lab
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Solexa Paired-End Library Construction
UW Stam Lab
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TruSeq Library Construction (non-Illumina protocol)
UW Stam Lab
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